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tic and phosphomolybdic acid, collagen is demonstrated by staining with aniline blue. Rinsing in acetic acid after staining renders the shades of color more delicate and transparent.4 Included is a. Massons Trichrome Staining (MTS) is a special histological staining method used to differentiate the connective tissue elements of a section. Massons Trichrome staining is the most robust technique to evaluate tissue fibrosis. As the name indicates, trichrome staining utilizes three dyes that stain nuclei, cytoplasm, and connective. Transfer sections directly (without rinse) to aniline blue solution and stain for 5-10 minutes. Rinse briefly in distilled water and differentiate in 1% acetic acid solution for 2-5 minutes.